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KMID : 1195620080010020080
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Clinical and Experimental Otorhinolaryngology
2008 Volume.1 No. 2 p.80 ~ p.85
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Apoptotic Pattern of Cochlear Outer Hair Cells and Frequency-specific Hearing Threshold Shift in Noise-exposed BALB/c Mice
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Lim Hyun-Woo
Lyu Seung-Hyo
Kang Hun-Hee
Ahn Joong-Ho
Chung Jong-Woo
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Abstract
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Objectives: Apoptosis of outer hair cell (OHC) can be identified through nuclear staining by specific nuclear changes. The change of filamentous actin (F-actin) is also involved in early cell death process. The study was designed to investigate OHC death along the whole length of the organ of Corti.
Methods: BALB/c hybrid mice were used in this study. The noise group was exposed to white noise of 120 dB SPL for 3 hr per day for 3 consecutive days. The tone burst auditory brainstem response (ABR) test was conducted and
cochleas from each group were obtained for the immunostaining of FITC phalloidin for F-actin and propidium iodide(PI) for nuclei.
Results: ABR threshold of the noise group significantly increased after noise exposure (P<0.001). No threshold shift was found in the control group. Threshold shift of the noise group constantly increased from 4 to 16 kHz, but threshold
shifts at 16 kHz and 32 kHz were similar. Patterns of OHC staining were subclassified as FITC+PI- cells, FITC+PI+ cells, FITC-PI+ cells and missing cells. Proportion of normal live OHCs (FITC+PI-) rapidly decreased from the apex to the base. In the basal turn, FITC-PI+ cells and vacancy OHC (missing cells) were observed easily. Apoptotic and missing cells were most abundant at 60% of the whole length of the Corti organ.
Conclusion: We could subclassify morphologic changes in OHC death after noise exposure. Quantitative changes in OHCs along the whole Corti organ showed a plateau pattern similar to that of a frequency-specific threshold shift.
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KEYWORD
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Noise-induced hearing loss, Apoptosis, Auditory brainstem evoked potential, Cell death
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